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KMID : 0362319970220020761
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Journal of Korean Academy of Operative Dentistry
1997 Volume.22 No. 2 p.761 ~ p.768
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EFFECT OF CULTURE SUPERNATANT OF BACTERIA ISOLATED FROM INFECTED ROOT CANALS ON CELL LINES
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임미경/Mi Kyung Im
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Abstract
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Microorganisms and their by-products are considered to be the major causes of pulpal
and periapical pathosis. The role of microorganisms in endodotic infection has been
studied regarding the prevalence of particular organisms found in root canal and
periapical lesions.
The aim of this study was to investigate the effect of culture supernatants of several
oral microorganims isolated from infected root canals on the viability of cultured cell
lines using colorimetirc assay.
S. simulans, S. sciuri, E. faecium, S. intermedius, S. mitis, S. sanguis and S. uberis
were incubated in Todd-Hewitt broth for 16 hours. 20 and 100ul of filtered bacterial cell
culture supernatants were added to MK and Hep-2 cells. Cell viability was measured
using MTT colorimetric assay. 20ul and 100ul of S. sanguis supernatant significantly
depressed viability of HEp-2 cells. E. faecium and S. intermedius did not affect the
viability of MK and HEp-2 cells.
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KEYWORD
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